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KMID : 0380219940270010047
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Journal of Biochemistry and Molecular Biology
1994 Volume.27 No. 1 p.47 ~ p.50
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Use of Randomly Amplified Polymorphic DNAs for Molecular Taxonomic Analysis in the Genus Brassica
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Abstract
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Abstract:
@EN The potential use of RAPDs (randomly amplified polymorphic DNAs) to detect genetic polymorphism among genera and species was investigated using six species of Brassica and one species of Raphanus. Ten arbitrary primers giving rise to 97 RAPD
bands
were enough to reveal differences among genotypes and it was possible to establish a phylogenic relationship among the taxa studied. A phenogram bases on Jaccard similarity coefficient comparing pair-wise unique and shared polymorphic
amplification
products confirmed the previously established U triangle relationship among rhe Brassica taxa. Furthermore, it was possible to establish a more detailed analysis of the relationship revealing a high degree of similarity between B. oleracea ( C )
and B.
(AC), and separation of b. juncea (AB) from B. campestris(A); analyses which were not possible using either restrictin fragment length polymorphism (RFLP) or isozyme marker analysis. RAPD analysis appears to be an efficient alternative to RFLP
and
isozyme methods and can be useful for more detailed taxonomic studies at the generic and specific levels.
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KEYWORD
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